The 24-year-old was in the running for a spot in England's 23-man squad, having enjoyed a fine season with Saints in which he netted 17 goals.
Such form saw Rodriguez handed his Three Lions debut in November's friendly against Chile, but his hopes of a spot on the plane to Brazil have been cruelly taken away.
The forward landed awkwardly during Saturday's 4-1 defeat at Manchester City and Southampton confirmed he has ruptured his anterior cruciate ligament.
Source : PA
Source: PA