He suffered an ankle injury after a heavy challenge and Saints are now awaiting an assessment of the problem.
Saints boss Nigel Adkins told BBC Radio Solent: "We'll take all the right precautions with Alex, because he's a very important player for us."
Chamberlain has been continually linked with a move to the Premier League in recent months, but Adkins says nothing has changed and that he expects the teenager to remain a Southampton player.
He said: "The speculation has been going on for a while, and we can't do anything about it.
"But he's put in some great performances in pre-season. Alex is very professional in what he does, and that will continue."
Source: BBC Sport
Source: BBC Sport